Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.
Identifieur interne : 005792 ( Main/Exploration ); précédent : 005791; suivant : 005793Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.
Auteurs : Moorthy Sakthidevi [Inde] ; Vadivel Murugan ; Sugeerappa Laxmanappa Hoti ; Perumal KalirajSource :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN des helminthes (génétique), Animaux, Antigènes d'helminthe (génétique), Brugia malayi (génétique), Diagnostic différentiel, Filariose lymphatique (diagnostic), Filariose lymphatique (parasitologie), Introns (génétique), Marqueurs génétiques (génétique), Modèles génétiques, Protéines recombinantes (génétique), Réaction de polymérisation en chaîne, Spécificité d'espèce, Séquences répétées en tandem, Wuchereria bancrofti (génétique).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : ADN des helminthes, Antigènes d'helminthe, Brugia malayi, Introns, Marqueurs génétiques, Protéines recombinantes, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- Animaux, Diagnostic différentiel, Modèles génétiques, Réaction de polymérisation en chaîne, Spécificité d'espèce, Séquences répétées en tandem.
English descriptors
- KwdEn :
- Animals, Antigens, Helminth (genetics), Brugia malayi (genetics), DNA, Helminth (genetics), Diagnosis, Differential, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (parasitology), Genetic Markers (genetics), Introns (genetics), Models, Genetic, Polymerase Chain Reaction, Recombinant Proteins (genetics), Species Specificity, Tandem Repeat Sequences, Wuchereria bancrofti (genetics).
- MESH :
- chemical , genetics : Antigens, Helminth, DNA, Helminth, Genetic Markers, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- genetics : Brugia malayi, Introns, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Animals, Diagnosis, Differential, Models, Genetic, Polymerase Chain Reaction, Species Specificity, Tandem Repeat Sequences.
Abstract
Polymerase chain reaction based methods are promising tools for the monitoring and evaluation of the Global Program for the Elimination of Lymphatic Filariasis. The currently available PCR methods do not differentiate the DNA of Wuchereria bancrofti or Brugia malayi by a single PCR and hence are cumbersome. Therefore, we designed a single step PCR strategy for differentiating Bancroftian infection from Brugian infection based on a newly identified gene from the W. bancrofti genome, abundant larval transcript-2 (alt-2), which is abundantly expressed. The difference in PCR product sizes generated from the presence or absence of evolutionarily altered tandem repeats in alt-2 intron-3 differentiated W. bancrofti from B. malayi. The analysis was performed on the genomic DNA of microfilariae from a number of patient blood samples or microfilariae positive slides from different Indian geographical regions. The assay gave consistent results, differentiating the two filarial parasite species accurately. This alt-2 intron-3 based PCR assay can be a potential tool for the diagnosis and differentiation of co-infections by lymphatic filarial parasites.
DOI: 10.1016/j.meegid.2010.01.012
PubMed: 20132908
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002B73
- to stream PubMed, to step Curation: 002B73
- to stream PubMed, to step Checkpoint: 002B73
- to stream Ncbi, to step Merge: 003905
- to stream Ncbi, to step Curation: 003905
- to stream Ncbi, to step Checkpoint: 003905
- to stream Main, to step Merge: 005842
- to stream Main, to step Curation: 005792
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.</title><author><name sortKey="Sakthidevi, Moorthy" sort="Sakthidevi, Moorthy" uniqKey="Sakthidevi M" first="Moorthy" last="Sakthidevi">Moorthy Sakthidevi</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Guindy, Chennai, Tamil Nadu 600025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Guindy, Chennai, Tamil Nadu 600025</wicri:regionArea><wicri:noRegion>Tamil Nadu 600025</wicri:noRegion></affiliation></author><author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name></author><author><name sortKey="Hoti, Sugeerappa Laxmanappa" sort="Hoti, Sugeerappa Laxmanappa" uniqKey="Hoti S" first="Sugeerappa Laxmanappa" last="Hoti">Sugeerappa Laxmanappa Hoti</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20132908</idno><idno type="pmid">20132908</idno><idno type="doi">10.1016/j.meegid.2010.01.012</idno><idno type="wicri:Area/PubMed/Corpus">002B73</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B73</idno><idno type="wicri:Area/PubMed/Curation">002B73</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002B73</idno><idno type="wicri:Area/PubMed/Checkpoint">002B73</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B73</idno><idno type="wicri:Area/Ncbi/Merge">003905</idno><idno type="wicri:Area/Ncbi/Curation">003905</idno><idno type="wicri:Area/Ncbi/Checkpoint">003905</idno><idno type="wicri:Area/Main/Merge">005842</idno><idno type="wicri:Area/Main/Curation">005792</idno><idno type="wicri:Area/Main/Exploration">005792</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.</title><author><name sortKey="Sakthidevi, Moorthy" sort="Sakthidevi, Moorthy" uniqKey="Sakthidevi M" first="Moorthy" last="Sakthidevi">Moorthy Sakthidevi</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Guindy, Chennai, Tamil Nadu 600025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Guindy, Chennai, Tamil Nadu 600025</wicri:regionArea><wicri:noRegion>Tamil Nadu 600025</wicri:noRegion></affiliation></author><author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name></author><author><name sortKey="Hoti, Sugeerappa Laxmanappa" sort="Hoti, Sugeerappa Laxmanappa" uniqKey="Hoti S" first="Sugeerappa Laxmanappa" last="Hoti">Sugeerappa Laxmanappa Hoti</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Helminth (genetics)</term><term>Brugia malayi (genetics)</term><term>DNA, Helminth (genetics)</term><term>Diagnosis, Differential</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Genetic Markers (genetics)</term><term>Introns (genetics)</term><term>Models, Genetic</term><term>Polymerase Chain Reaction</term><term>Recombinant Proteins (genetics)</term><term>Species Specificity</term><term>Tandem Repeat Sequences</term><term>Wuchereria bancrofti (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (génétique)</term><term>Animaux</term><term>Antigènes d'helminthe (génétique)</term><term>Brugia malayi (génétique)</term><term>Diagnostic différentiel</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (parasitologie)</term><term>Introns (génétique)</term><term>Marqueurs génétiques (génétique)</term><term>Modèles génétiques</term><term>Protéines recombinantes (génétique)</term><term>Réaction de polymérisation en chaîne</term><term>Spécificité d'espèce</term><term>Séquences répétées en tandem</term><term>Wuchereria bancrofti (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>DNA, Helminth</term><term>Genetic Markers</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Introns</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des helminthes</term><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Introns</term><term>Marqueurs génétiques</term><term>Protéines recombinantes</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Diagnosis, Differential</term><term>Models, Genetic</term><term>Polymerase Chain Reaction</term><term>Species Specificity</term><term>Tandem Repeat Sequences</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Diagnostic différentiel</term><term>Modèles génétiques</term><term>Réaction de polymérisation en chaîne</term><term>Spécificité d'espèce</term><term>Séquences répétées en tandem</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polymerase chain reaction based methods are promising tools for the monitoring and evaluation of the Global Program for the Elimination of Lymphatic Filariasis. The currently available PCR methods do not differentiate the DNA of Wuchereria bancrofti or Brugia malayi by a single PCR and hence are cumbersome. Therefore, we designed a single step PCR strategy for differentiating Bancroftian infection from Brugian infection based on a newly identified gene from the W. bancrofti genome, abundant larval transcript-2 (alt-2), which is abundantly expressed. The difference in PCR product sizes generated from the presence or absence of evolutionarily altered tandem repeats in alt-2 intron-3 differentiated W. bancrofti from B. malayi. The analysis was performed on the genomic DNA of microfilariae from a number of patient blood samples or microfilariae positive slides from different Indian geographical regions. The assay gave consistent results, differentiating the two filarial parasite species accurately. This alt-2 intron-3 based PCR assay can be a potential tool for the diagnosis and differentiation of co-infections by lymphatic filarial parasites.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Hoti, Sugeerappa Laxmanappa" sort="Hoti, Sugeerappa Laxmanappa" uniqKey="Hoti S" first="Sugeerappa Laxmanappa" last="Hoti">Sugeerappa Laxmanappa Hoti</name><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name></noCountry><country name="Inde"><noRegion><name sortKey="Sakthidevi, Moorthy" sort="Sakthidevi, Moorthy" uniqKey="Sakthidevi M" first="Moorthy" last="Sakthidevi">Moorthy Sakthidevi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005792 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005792 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20132908
|texte= Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20132908" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |